Biochemical quantification of DNA in human articular and septal cartilage using PicoGreen® and Hoechst 33258
نویسندگان
چکیده
منابع مشابه
Biochemical quantification of DNA in human articular and septal cartilage using PicoGreen and Hoechst 33258.
OBJECTIVE To compare two fluorometric assays, utilizing (1) the bisbenzimidazole Hoechst 33258 and (2) PicoGreen, for determining DNA content in human cartilage. METHODS Human articular and nasal septal cartilage explants were digested using proteinase K. Portions of sample digest were analysed for intrinsic and dye-enhanced fluorescence with either Hoechst 33258 or PicoGreen. RESULTS Intri...
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ژورنال
عنوان ژورنال: Osteoarthritis and Cartilage
سال: 2002
ISSN: 1063-4584
DOI: 10.1053/joca.2002.0794